Product Pathways - MAPK Signaling
RSK2 (D21B2) XP® Rabbit mAb #5528
|5528S||100 µl (10 western blots)||---||In Stock||---|
|5528P||40 µl (4 western blots)||---||In Stock||---|
|5528||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey, Bovine, Pig||Endogenous||90||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Dog, Horse.
Specificity / Sensitivity
RSK2 (D21B2) XP® Rabbit mAb recognizes endogenous levels of total RSK2 protein. This antibody does not cross-react with other RSK isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro686 of human RSK2 protein.
Western blot analysis of extracts from various cell lines using RSK2 (D21B2) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using RSK2 (D21B2) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RSK2 (D21B2) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using RSK2 (D21B2) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcinoma using RSK2 (D21B2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of HeLa cells using RSK2 (D21B2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with sodium arsenite (500 uM for 1 hr; right) using RSK2 (D21B2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Localization of RSK2 to "stress granules" in arsenite-treated cells is very similar to that seen by Eisinger-Mathason et. al. (Molecular Cell, Volume 1, 5 September 2008, Pages 722-736).
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with insulin, EGF, and serum (right), using RSK2 (D21B2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of recombinant GST-tagged RSK1, RSK2, and RSK3 (2 μg each) using RSK1 (D6D5) Rabbit mAb #8408 (left panel), RSK2 (D21B2) XP® Rabbit mAb #5528 (center panel), or GST (91G1) Rabbit mAb #2625 (right panel).
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
Stimulation by various growth factors leads to activation of RSK2, which is a critical downstream effector kinase in several pathways. EGF stimulation leads to phosphorylation of CREB at Ser133 and phosphorylation of histone H3 in vivo by RSK2 (4,5). RSK2 phosphorylation of p53 may help regulate chromatin structure and cell cycle (6). RSK2 is prominently expressed in the brain and is essential for cognitive function and learning. During development, RSK2 regulates the differentiation of osteoblasts and skeletal muscle cells (7,8). Mutations in the corresponding gene are associated with Coffin-Lowry syndrome (CLS), an X-linked disorder characterized by mental retardation and the presence of characteristic facial anomalies (9).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- De Cesare, D. et al. (1998) Proc Natl Acad Sci U S A 95, 12202-7.
- Sassone-Corsi, P. et al. (1999) Science 285, 886-91.
- Cho, Y.Y. et al. (2005) Cancer Res 65, 3596-603.
- Yang, X. et al. (2004) Cell 117, 387-98.
- Cho, Y.Y. et al. (2007) J Biol Chem 282, 8380-92.
- Delaunoy, J.P. et al. (2006) Clin Genet 70, 161-6.
- Fujiki, K. et al. (2013) Arch Toxicol , . Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8112 SignalStain® Antibody Diluent
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 8408 RSK1 (D6D5) Rabbit mAb
- 9340 RSK2 Antibody
- 9333 RSK1 Antibody
- 9343 RSK3 Antibody
- 9347 RSK1/RSK2/RSK3 Antibody
- 3556 Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
- 9326 p90RSK Antibody Sampler Kit
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.