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Render Timestamp: 2024-10-31T10:36:21.069Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:58:43.495
Product last modified at: 2024-09-30T08:00:48.062Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. TRIB1 (E7N2N) Mouse mAb

TRIB1 (E7N2N) Mouse mAb #58801

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Mouse IgG2b kappa
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRIB1 (E7N2N) Mouse mAb recognizes endogenous levels of total TRIB1 protein. This antibody is not predicted to cross-react with other TRIBBLES proteins, based on sequence divergence in the epitope region.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro344 of human TRIB1 protein.

    Background

    TRIBBLES proteins belong to a small family of serine-threonine kinase-like proteins characterized by the presence of a variant protein kinase motif (lacking a canonical ATP binding site), a MEK1 binding site, and a C-terminal COP1 site that binds ubiquitin ligase. The tribbles gene was first identified and characterized in Drosophila genetic screens for genes that regulate cell division, gastrulation, and oogenesis (1-3). Research studies in Drosophila suggested that TRIBBLES functions to coordinate cell division by regulating turnover of the cell cycle protein String/cdc25. In contrast to the Drosophila genome, which contains a single tribbles gene, the genomes of mice and humans encode three known TRIBBLES proteins (TRIB1-3), which exhibit both distinct and overlapping patterns of expression and functions (4). For example, TRIB1 and TRIB2, but not TRIB3, were reported to promote degradation of the basic region-leucine zipper transcription factor C/EBPα, a function that appears to be conserved from flies to humans (5,6). TRIB2 is overexpressed in a subset of human AML patient samples, downregulated in leukemic cells undergoing proliferation arrest (7), and positively regulated by the NOTCH signaling pathway in T cells (8), while retroviral-mediated overexpression of TRIB2 in mice was shown to induce transplantable leukemia (7). These findings collectively suggest that TRIB2 functions as an oncogene in the mammalian hematopoietic system (9).

    Database Links

    UniProt ID: Q96RU8


    Entrez-Gene Id: 10221


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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