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CENP-F (D6X4L) Rabbit Monoclonal Antibody #58982

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  • WB
  • IF
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 450
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #57905.

    Protocol

    Specificity / Sensitivity

    CENP-F (D6X4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CENP-F protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1794 of human CENP-F protein.

    Background

    CENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9).

    Alternate Names

    AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; CENPF; Centromere protein F; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa; CILD31; hcp-1; Kinetochore protein CENPF; Mitosin; PRO1779; STROMS

    For Research Use Only. Not for Use in Diagnostic Procedures.
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