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NDP52 (D1E4A) Rabbit Monoclonal Antibody #60732

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 52, 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NDP52 (D1E4A) Rabbit Monoclonal Antibody recognizes endogenous levels of total NDP52 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val135 of human NDP52 protein.

    Background

    NDP52 (CALCOCO2) is ubiquitously expressed and composed of an amino-terminal SKICH domain, followed by a coiled-coil domain, and two zinc finger domains (1). It has recently been reported to act as an autophagy receptor that binds cytosolic ubiquitinated bacteria, leading to autophagy activation and pathogen clearance (1-3). NDP52 binds ubiquitin through its zinc finger domain and simultaneously binds LC3, which directs the bacteria into autophagosomes. In addition, NDP52 interacts with Nap1 and SINTBAD to recruit TBK1 to ubiquitinated bacteria (1).

    Alternate Names

    Antigen nuclear dot 52 kDa protein; CACO2; calcium binding and coiled-coil domain 2; Calcium-binding and coiled-coil domain-containing protein 2; CALCOCO2; MGC17318; NDP52; Nuclear domain 10 protein 52; Nuclear domain 10 protein NDP52; Nuclear dot protein 52

    For Research Use Only. Not for Use in Diagnostic Procedures.
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