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  3. TRAIL (C92B9) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 647 Conjugate)

TRAIL (C92B9) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 647 Conjugate) #61506

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #3219

    Fluorescent Properties

    • ← Excitation: 650 nm ← Emission: 668 nm

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    TRAIL (C92B9) Rabbit mAb (SignalFlex™ Alexa Fluor® 647 Conjugate) detects endogenous levels of total human TRAIL protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys60 of human TRAIL, within the extracellular region of the protein.

    Background

    Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (6,8,10,11) and DcR2 (TRAIL-R4, TRUNDD) (12,13) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (14). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (15,16).
    1. Wiley, S.R. et al. (1995) Immunity 3, 673-82.
    2. Pitti, R.M. et al. (1996) J Biol Chem 271, 12687-90.
    3. Almasan, A. and Ashkenazi, A. Cytokine Growth Factor Rev 14, 337-48.
    4. Pan, G. et al. (1997) Science 276, 111-3.
    5. Walczak, H. et al. (1997) EMBO J 16, 5386-97.
    6. MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20.
    7. Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
    8. Schneider, P. et al. (1997) FEBS Lett 416, 329-34.
    9. Shetty, S. et al. (2002) Apoptosis 7, 413-20.
    10. Sheridan, J.P. et al. (1997) Science 277, 818-21.
    11. Degli-Esposti, M.A. et al. (1997) J Exp Med 186, 1165-70.
    12. Pan, G. et al. (1998) FEBS Lett 424, 41-5.
    13. Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
    14. Kelley, S.K. et al. (2001) J Pharmacol Exp Ther 299, 31-8.
    15. Walczak, H. et al. (1999) Nat Med 5, 157-63.
    16. Ashkenazi, A. et al. (1999) J Clin Invest 104, 155-62.

    Alternate Names

    Apo-2 ligand; Apo-2L; APO2L; CD253; chemokine tumor necrosis factor ligand superfamily member 10; Protein TRAIL; TL2; TNF superfamily member 10; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; TNF10; TNFSF10; TNLG6A; TRAIL; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; Tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10

    Database Links

    UniProt ID: P50591


    Entrez-Gene Id: 8743


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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