HMCES (F4E4Q) Rabbit Monoclonal Antibody #65616
- WB
- IP
- IF
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
During somatic hypermutation (SHM) in B cells, HMCES suppresses deletions within immunoglobulin (Ig) genes but allows other types of point mutations to occur, resulting in antigen-specific high-affinity antibodies (4). HMCES deficiency impairs class switch recombination (CSR) in B cells, leading to weakened antibody production (5).
In APOBEC3A-expressing tumors, disruption of HMCES may increase the tumor’s sensitivity to therapies such as ionizing radiation (IR), oxidative stress, and ATR inhibition (6).
- Amidon, K.M. and Eichman, B.F. (2020) DNA Repair (Amst) 94, 102903.
- Mohni, K.N. et al. (2019) Cell 176, 144-153.e13.
- Rua-Fernandez, J. et al. (2023) Cell Rep 42, 113427.
- Wu, L. et al. (2022) Genes Dev 36, 433-450.
- Shukla, V. et al. (2020) Mol Cell 77, 384-394.e4.
- Biayna, J. et al. (2021) PLoS Biol 19, e3001176.
Alternate Names
5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine binding, ES cell specific; Abasic site processing protein HMCES; C3orf37; DC12; Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; HMCES; MGC111075; Peptidase HMCES; putative endonuclease HMCES; putative peptidase SRAPD1; SOS response associated peptidase domain containing 1; SRAP domain-containing protein 1; SRAPD1; UPF0361 protein C3orf37
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.