PathScan® Total Chk2 Sandwich ELISA Kit

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ELISA

Inquiry Info. # 7045

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Product Specifications

REACTIVITY

H

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

The PathScan^® Total Chk2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Chk2. A Chk2 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, Chk2 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Chk2 mouse detection antibody is added to the captured phospho and nonphospho Chk2 protein. Anti-mouse IgG, HRP-linked Antibody #7076 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total Chk2.
Antibodies in kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan^® Total Chk2 Sandwich ELISA Kit #7045 detects endogenous levels of total Chk2 protein (see Figure 1). This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

Alternate Names

Cds1; Cds1 homolog; Checkpoint kinase 2; checkpoint-like protein CHK2; CHEK2; CHK2; CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); hCds1; HuCds1; LFS2; PP1425; protein kinase CHK2; RAD53; Serine/threonine-protein kinase Chk2

Database Links

UniProt ID: O96017

Entrez-Gene Id: 11200

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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PathScan® Total Chk2 Sandwich ELISA Kit #7045