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  3. PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Antibody Pair

PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Antibody Pair #7343

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  • ELISA

Inquiry Info. # 7343

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Storage

    Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

    Protocol

    Product Description

    CST's PathScan® Phospho-IκB-α (Ser32) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Kit #7355. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IκBα Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-IκBα (Ser32) Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-IκBα (Ser32) protein.
    Antibodies in kit are custom formulations specific to kit.

    Specificity / Sensitivity

    For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

    Alternate Names

    IκB-α; IκBα

    Database Links

    UniProt ID: P25963


    Entrez-Gene Id: 4792


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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