Render Target: STATIC
Render Timestamp: 2024-10-31T10:02:18.379Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-06-11 16:01:22.204
Product last modified at: 2024-10-28T11:45:46.150Z
1% for the planet logo
PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Apoptosis/Necroptosis Antibody Sampler Kit II #73966

    Product Information

    Product Description

    The Apoptosis/Necroptosis Antibody Sampler Kit II provides an economical means of detecting markers for apoptosis and necroptosis. The kit contains enough primary antibody to perform at least two western blot experiments.

    Background

    Apoptosis is a regulated physiological process leading to cell death (1,2). Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Caspases are synthesized as inactive zymogens containing a pro-domain followed by large (p20) and small subunits (p10) that are proteolytically processed in a cascade of caspase activity. Initiator caspases (including 8, 9, 10, and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6, and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF, and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase. Apoptosis induced through the extrinsic mechanisms involving death receptors in the tumor necrosis factor receptor superfamily activates caspase-8. Activated caspase-8 cleaves and activates downstream effector caspases, such as caspase-1, -3, -6, and -7. Caspase-3 is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP).

    Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals, including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), and ischemic injury (3,4). Necroptosis is negatively regulated by caspase-8 mediated apoptosis in which the kinase RIP/RIPK1 is cleaved (5). Furthermore, necroptosis is inhibited by a small molecule inhibitor of RIP, necrostatin-1 (Nec-1) (6). Research studies show that necroptosis contributes to a number of pathological conditions, and Nec-1 has been shown to provide neuroprotection in models such as ischemic brain injury (7). RIP is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166 (8). Phosphorylation drives association with RIP3, which is required for necroptosis (9-11). Mixed lineage kinase domain-like protein (MLKL) is a pseudokinase that was identified as a downstream target of RIP3 in the necroptosis pathway (12). During necroptosis, RIP3 is phosphorylated at Ser227, which recruits MLKL and leads to its phosphorylation at Thr357 and Ser358 (12). Knockdown of MLKL through multiple mechanisms results in inhibition of necroptosis (13). Phosphorylation of MLKL during necroptosis leads to its oligomerization with pore formation that affects membrane integrity (14-17).
    1. Degterev, A. et al. (2003) Oncogene 22, 8543-67.
    2. Green, D.R. (1998) Cell 94, 695-8.
    3. Christofferson, D.E. and Yuan, J. (2010) Curr Opin Cell Biol 22, 263-8.
    4. Kaczmarek, A. et al. (2013) Immunity 38, 209-23.
    5. Lin, Y. et al. (1999) Genes Dev 13, 2514-26.
    6. Degterev, A. et al. (2008) Nat Chem Biol 4, 313-21.
    7. Degterev, A. et al. (2005) Nat Chem Biol 1, 112-9.
    8. Ofengeim, D. and Yuan, J. (2013) Nat Rev Mol Cell Biol 14, 727-36.
    9. Cho, Y.S. et al. (2009) Cell 137, 1112-23.
    10. He, S. et al. (2009) Cell 137, 1100-11.
    11. Zhang, D.W. et al. (2009) Science 325, 332-6.
    12. Sun, L. et al. (2012) Cell 148, 213-27.
    13. Wu, J. et al. (2013) Cell Res 23, 994-1006.
    14. Cai, Z. et al. (2014) Nat Cell Biol 16, 55-65.
    15. Chen, X. et al. (2014) Cell Res 24, 105-21.
    16. Wang, H. et al. (2014) Mol Cell 54, 133-46.
    17. Dondelinger, Y. et al. (2014) Cell Rep 7, 971-81.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.