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SARS-CoV-1 Membrane Protein Antibody #74471

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  • WB

    Product Specifications

    REACTIVITY Vir
    SENSITIVITY Transfected Only
    MW (kDa) 20-30
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SARS-CoV-1 Membrane Protein Antibody recognizes transfected levels of total SARS-CoV-1 membrane protein. This antibody does not cross-react with SARS-CoV-2 or MERS-CoV membrane proteins.

    Species Reactivity:

    Virus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly211 of SARS-CoV-1 membrane protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of single-stranded RNA viruses (2). The genome of SARS-CoV-1 is relatively large among viruses (~30 kDa); it encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to a variety of smaller open reading frames (ORFs) that encode the accessory proteins required for viral replication (3). The SARS-CoV-1 membrane protein (M) is the most abundant protein in the viral envelope; in addition to its structural role, it plays an important role in virus-host interactions and the viral lifecycle (4). Notably, studies have shown that membrane proteins from both SARS-CoV-1 and SARS-CoV-2 may induce apoptosis in host cells, suggesting one mechanism by which SARS coronaviruses cause tissue damage upon infection (5,6).

    Alternate Names

    E1 glycoprotein; M protein; Matrix glycoprotein; matrix protein; Membrane glycoprotein; membrane glycoprotein M; Membrane protein; SARS-CoV membrane protein; sars5; VME1_CVHSA

    For Research Use Only. Not for Use in Diagnostic Procedures.
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