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  3. PathScan® Total IRS-2 Sandwich ELISA Kit

PathScan® Total IRS-2 Sandwich ELISA Kit #7884

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Inquiry Info. # 7884

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    Product Specifications

    REACTIVITY M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    The PathScan® Total IRS-2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total IRS-2 (also called 4PS). An IRS-2 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, IRS-2 (both phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a biotinylated IRS-2 rabbit antibody is added to detect the captured IRS-2. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate (TMB) is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2.
    Antibodies in kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    PathScan® Total IRS-2 Sandwich ELISA Kit #7884 detects endogenous levels of IRS-2. As shown in Figure 1, using this Sandwich ELISA Kit #7884, a high level of IRS-2 is detected in transfected CHO cells. The total levels of IRS-2 (phospho and nonphospho) remain unchanged after insulin treatment as shown by western analysis. This kit also detects endogenous levels of IRS-2 expressed by 3T3-L1 adipocytes (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Mouse

    Background

    Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

    Alternate Names

    Insulin receptor substrate 2; IRS-2; IRS2

    Database Links

    UniProt ID: Q9Y4H2


    Entrez-Gene Id: 8660


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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