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Met (D1C2) Rabbit Monoclonal Antibody (Biotinylated)

Met (D1C2) Rabbit Monoclonal Antibody (Biotinylated) #8041

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	140, 170
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology^® antibody is the biotinylated version of the unconjugated Met (D1C2) XP^® Rabbit mAb #8198 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. (The following statement only for reformulated conjugates with WB image) Western blot data were generated using the unconjugated antibody.

MW (kDa)

140, 170

Product Usage Information

Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.

Storage

Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Met (D1C2) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total Met protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Met protein.

Background

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).

Alternate Names

AUTS9; c-Met; DFNB97; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; HGFR; MET; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET proto-oncogene, receptor tyrosine kinase; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

Database Links

UniProt ID: P08581

Entrez-Gene Id: 4233

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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