Product Pathways - Tyrosine Kinase / Adaptors
FGF Receptor 4 (D3B12) XP® Rabbit mAb #8562
|8562S||100 µl (10 western blots)||---||In Stock||---|
|8562P||40 µl (4 western blots)||---||In Stock||---|
|8562||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||95, 125||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
FGF Receptor 4 (D3B12) XP® Rabbit mAb detects endogeneous levels of total FGF receptor 4 protein. This antibody may slightly cross-react with overexpressed FGF Receptor family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser792 of human FGF receptor 4 protein.
Western blot analysis of extracts from various cell lines using FGF Receptor 4 (D3B12) XP® Rabbit mAb. (Lower molecular weight degradation products of FGF receptor 4 are present in some cell lines.)
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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