Render Target: STATIC
Render Timestamp: 2024-10-31T10:54:04.838Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-20 06:18:55.795
Product last modified at: 2024-09-26T11:45:38.637Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

QKI Antibody #86397

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 38, 42
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    QKI Antibody recognizes endogenous levels of total QKI protein. This antibody detects QKI-5 and other isoforms.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly202 of human QKI protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Quaking (QKI) is a member of the signal transduction and activator of RNA (STAR) protein family of RNA-binding proteins (1,2). Mutations in the mouse Qki locus results in impaired myelin formation resulting in tremors (3). QKI proteins exist as homodimers, and disruption of the dimerization process is lethal in mice (2). QKI exists in different isoforms that differ in their C-terminus, resulting in unique subcellular localizations (4). The nuclear isoform of QKI, QKI-5, is involved in regulation of alternative splicing of MAG mRNA, which encodes for a protein important for myelin sheath formation and maintenance (4,5). QKI has also been implicated in schizophrenia and oligodendrocyte differentiation (6). QKI can function as a tumor suppressor, as it is regulated by p53 to stabilize miRNAs that regulate TGF-β signaling (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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