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Lamin A (133A2) Mouse Monoclonal Antibody #86846

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 74
    Source/Isotype Mouse IgG3
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:500 - 1:2000
    Immunofluorescence (Immunocytochemistry) 1:12800 - 1:25600

    Storage

    Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C.

    Protocol

    Specificity / Sensitivity

    Lamin A (133A2) Mouse Monoclonal Antibody recognizes endogenous levels of total lamin A protein. This antibody also reacts with large fragments of lamin A (50 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamin C.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human lamin A protein.

    Background

    Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).

    Alternate Names

    70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C; lamin A/C-like 1; Lamin-A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; Prelamin-A/C; PRO1; Renal carcinoma antigen NY-REN-32

    For Research Use Only. Not for Use in Diagnostic Procedures.
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