Product Pathways - Vesicle Trafficking
LAMP1 (D2D11) XP® Rabbit mAb #9091
|9091S||100 µl (10 western blots)||---||In Stock||---|
|9091P||40 µl (4 western blots)||---||In Stock||---|
|9091||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||42 (non-glycosylated), 90-120 (glycosylated)||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
* Product-specific protocol.
Specificity / Sensitivity
LAMP1 (D2D11) XP® Rabbit mAb recognizes endogenous levels of total LAMP1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein.
Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.
Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).
- Eskelinen, E.L. et al. (2003) Trends Cell Biol 13, 137-45.
- Fukuda, M. (1991) J Biol Chem 266, 21327-30.
- Kundra, R. and Kornfeld, S. (1999) J Biol Chem 274, 31039-46.
- Rohrer, J. et al. (1996) J Cell Biol 132, 565-76.
- Huynh, K.K. et al. (2007) EMBO J 26, 313-24.
- Eskelinen, E.L. et al. (2004) Mol Biol Cell 15, 3132-45.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.