Product Pathways - Jak/Stat Pathway
Stat1 (9H2) Mouse mAb #9176
|9176S||100 µl (10 western blots)||---||In Stock||---|
|9176||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||84, 91||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Bovine.
Specificity / Sensitivity
Stat1 (9H2) Mouse mAb detects endogenous levels of total Stat1 protein independent of phosphorylation, however, it prefers the non-phosphorylated form of Stat1. The antibody detects both Stat1alpha (91 kDa) and Stat1beta (84 kDa) isoforms. It does not significantly cross-react with the other Stat proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide.
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 minutes), using Stat1 (9H2) Mouse mAb.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
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