Product Pathways - MAPK Signaling
ATF-2 Control Cell Extracts #9223
|9223S||200 µl (10 western blots)||---||In Stock||---|
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Nonphosphorylated ATF-2 Control Cell Extracts: Total extracts from NIH/3T3 cells, prepared without treatment, to serve as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC.
Phosphorylated ATF-2 Control Cell Extracts: Total extracts from NIH/3T3 cells, prepared with anisomycin treatment, to serve as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.
Western Blots: For controls, we recommend using 20 µl of phosphorylated and nonphosphorylated ATF-2 control extracts.
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
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