Product Pathways - Apoptosis
Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) #9429
|9429S||100 µl (10 western blots)||---||In Stock||---|
|9429||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Rat.
Specificity / Sensitivity
Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to the Asp387 cleavage site of rodent caspase-8. Antibodies were purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from COS cells, mock transfected (-) or transfected with a construct overexpressing mouse caspase-8 (+), using Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific).
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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