Product Pathways - Apoptosis
Caspase-7 Antibody #9492
|9492S||100 µl (10 western blots)||---||In Stock||---|
|9492P||40 µl (4 western blots)||---||In Stock||---|
|9492||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||20, 35||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Caspase-7 Antibody detects endogenous levels of both full length caspase-7 (35 kDa) and the large fragment of cleaved caspase-7 following cleavage at aspartic acid 198 (20 kDa). The antibody does not recognize other caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography.
Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).
- Erhardt, J. A. et al. (2001) Thromb. Res 103, 143-148. Applications: Western Blotting.
- Lademann, U. et al. (2003) Mol. Cell. Biol. 23, 7829-7837. Applications: Western Blotting.
- Guegan, C. et al. (2001) . J. Neurosci. 21, 6569-6576. Applications: Western Blotting.
- Thome, C.H. et al. (2012) Mol Cell Proteomics , . Applications: Western Blotting.
- Gucký, T. et al. (2013) J Med Chem 56, 6234-47. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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