Render Target: STATIC
Render Timestamp: 2024-10-31T10:00:09.021Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-20 06:19:38.246
Product last modified at: 2024-09-27T12:45:07.679Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Cleaved DFF45 (Asp224) Antibody #9731

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 12
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at aspartic acid 224. The antibody does not recognize full length DFF45 or other cleavage products of DFF45.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).
    (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
    For Research Use Only. Not For Use In Diagnostic Procedures.
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