Product Pathways - Adhesion
Cadherin-Catenin Antibody Sampler Kit #9961
|9961S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|α-E-Catenin (23B2) Rabbit mAb #3240||40 µl||W, IP||H, M, Mk||100||Rabbit IgG|
|N-Cadherin Antibody #4061||40 µl||W||H, M, R, Mk||Dg||140||Rabbit|
|E-Cadherin (24E10) Rabbit mAb #3195||40 µl||W, IHC-P, IHC-F, IF-IC, F||H, M||B, Dg, Pg||135||Rabbit IgG|
|P-Cadherin (C13F9) Rabbit mAb #2189||40 µl||W, IF-IC||H||Mk||120||Rabbit IgG|
|Pan-Cadherin (28E12) Rabbit mAb #4073||40 µl||W||H, M, R||130-150||Rabbit IgG|
|β-Catenin (D10A8) XP® Rabbit mAb #8480||40 µl||W, IP, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP||H, M, R, Mk||Z, B, Pg, GP, Hr||92||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat
Western blot analysis of extracts from various cell lines using P-Cadherin (C13F9) Rabbit mAb #2189.
Western blot analysis of extracts from various cell lines using E-Cadherin (24E10) Rabbit mAb #3195.
Western blot analysis of extracts from HUVEC, NIH/3T3, MCF-7, ZR-75 and A431 cells using α-E-Catenin (23B2) Rabbit mAb #3240.
Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.
Western blot analysis of extracts from various cell lines using Pan-Cadherin (28E12) Rabbit mAb #4073.
Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb #8480.
This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Specificity / Sensitivity
Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin (28E12) Rabbit mAb #4073 detects endogenous levels of total cadherin proteins.
Source / Purification
Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues within the amino-terminus of human N-cadherin and is purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin, residues near the carboxy terminus of human P-cadherin, residues surrounding 780 of human E-cadherin, residues surrounding Pro714 of human ß-catenin protein, and a synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
- Christofori, G. (2003) EMBO J. 22, 2318-2323.
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.