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Activators & Inhibitors
Chemical Modulators

A-769662 #64839

Citations (0)
Western blot analysis of extracts from PC-3 cells, untreated (-) or treated with A-769662 (1 mM, 1hr; +), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 (upper) or AMPKα Antibody #2532 (lower).
Chemical structure of A-769662.

Product Usage Information

A-769662 is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 924.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.


Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 360.4 g/mol
Purity >98%
Molecular Formula C20H12N2O3S
CAS 844499-71-4
Solubility Soluble in DMSO at 30 mg/ml or ethanol at 5 mg/ml with slight warming.


A-769662 is a potent activator of AMP-activated protein kinase (AMPK) by allosterically binding to the β and γ subunits and by inhibiting dephosphorylation of AMPK on Thr172 (1). This small molecule selectively targets AMPK heterotrimers containing the β1 subunit (2). A-769662 has been shown to stimulate purified rat liver AMPK (EC50 = 0.8 µM) and inhibit fatty acid synthesis in rat hepatocytes (IC50 = 3.2 µM) (3). Treatment of adipocytes with A-769662 inhibits cell differentiation, leads to over-activation of AMPK, and inactivation of acetyl-CoA carboxylase (ACC), making this an interesting compound when studying obesity (4). 

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 64839
Cat. # Size Qty. Price
5 mg