Name: Anisomycin
Price: 148.00 USD
Availability: InStock

Product Information

Product Usage Information

Anisomycin is supplied as a lyophilized powder. For a 25 mg/ml stock, reconstitute the 10 mg in 400 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg/ml for 5-60 minutes. Soluble in DMSO or MeOH.

Storage

Store lyophilized at -20C. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 1 month and store at -20C. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	265.3 g/mol
Purity	>98%
Molecular Formula	C₁₄H₁₉NO₄
CAS	22862-76-6
Solubility	Soluble in DMSO at 100mg/ml and MeOH at 50mg/ml.

Background

Anisomycin, an antibiotic produced by Streptomyces griseolus and S. roseochromogenes, was originally described to inhibit protein-protein synthesis at the translational level (1). More recently, it is has been well characterized to strongly activate the stress kinases SAPK/JNK and p38 MAPK, as well as p70/85 S6 kinase in mammalian cells, which results in the rapid induction of immediate-early (IE) genes, such as c-fos, fosB, c-jun, JunB, and JunD (1). Investigators have demonstrated that anisomycin acts as a potent signaling agonist, synergizing with growth factors and phorbol esters to superinduce these IE genes (1,2). Research studies have demonstrated that anisomycin induces apoptosis in many cancer cell lines (3-5).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Anisomycin #2222