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Ionomycin, Calcium Salt
Activators & Inhibitors
Chemical Modulators

Ionomycin, Calcium Salt #9995

Citations (0)
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Ionomycin (1 μM, 1 hr; right), using NFAT1 (D43B1) XP® Rabbit mAb #5861 (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Structure of Ionomycin

Product Usage Information

Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time.


Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 747.08 g/mol
Purity >99%
Molecular Formula C41H70O9 • Ca2+
CAS 56092-82-1
Solubility Soluble in DMSO at 1.6mg/ml and EtOH at 20mg/ml.


Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 9995
Cat. # Size Qty. Price
2.5 mg