Chemical structure of Ivermectin.
Ivermectin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 10 mg of powder in 1.14 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 875.1 g/mol
Purity: >98%, contains up to 10% B1b form.
Molecular Formula: C48H74O14 (B1a form)
Solubility: Soluble in DMSO at 70 mg/ml or ethanol at 25 mg/ml.
Ivermectin is a positive allosteric modulator of the ATP-activated ion channel, P2X purinoceptor 4 (P2X4), as well as the α7 neuronal nicotinic acetylcholine receptor (α7-nAChR) (1,2). The α7-nAChR is required for the cytokine suppressing mechanism of the inflammatory reflex and it stimulates activation of calcium ion transport. Impairment of the α7-nAChR can lead to overproduction of cytokines and a heightened inflammatory response (3). Ivermectin is a known anthelmintic agent, activating the glutamate-gated chloride channels in the nerves and muscles of parasites. The disruption of the parasite’s ingestive activity eventually leads to starvation (4). Ivermectin has been shown to inhibit SARS-CoV-2 replication in vitro with an IC50 value of ~2 μM (5).
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