Render Target: STATIC
Render Timestamp: 2024-11-05T09:50:00.849Z
Commit: 57f6e368eba1a427377652f2ad915d45d7f340a4
XML generation date: 2024-09-20 06:19:43.168
Product last modified at: 2024-10-29T14:15:09.715Z
1% for the planet logo
PDP - Template Name: Chemical Modulators
PDP - Template ID: *******c501c72

LY294002 #9901

    Product Information

    Product Usage Information

    For 10 mM stock, reconstitute 1.5 mg in 488 µl DMSO. For 50 mM stock, reconstitute 1.5 mg in 98 µl DMSO. Store aliquots at -20ºC.

    Storage

    Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 307.35 g/mol
    Purity >99%
    Molecular Formula C19H17NO3
    CAS 154447-36-6
    Solubility Soluble in DMSO and EtOH at 25mg/ml.

    Background

    LY294002 was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50 μM, it specifically abolished PI3 kinase activity (IC50=0.43 μg/ml; 1.40 μM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 is soluble in DMSO or ethanol. For use with in vitro or cell-based assays, it may be diluted into aqueous buffers to yield the desired concentrations. For experiments with cultured cells, CST recommends treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.