Chemical structure of Nelfinavir Mesylate.
Nelfinavir Mesylate is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 753 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 663.9 g/mol
Molecular Formula: C32H45N3O4S • CH3SO3H
Solubility: Soluble in DMSO at 70 mg/ml.
Nelfinavir Mesylate, also known as AG-1343, is a potent antiviral inhibitor of HIV-1 protease with a Ki value of 2 nM, in vitro (1). This small molecule is an effective antitumor agent, known to induce endoplasmic reticulum (ER) stress, autophagy, and apoptosis in vitro and in vivo (2). Nelfinavir Mesylate has anti-microbial effects on malaria, tuberculosis, and SARS (3). It has also been used in therapies as an antifibrotic agent against pulmonary fibrosis in patients with systemic sclerosis (4). Nelfinavir Mesylate is known to reduce inflammation by activating protein phosphatase 2 (PP2) and blocking MAPK signaling in macrophages (5). Through a novel mechanism of heat shock protein 90 (HSP90) inhibition, Nelfinavir Mesylate selectively inhibits the growth of HER2-positive breast cancer cells in vitro (6).
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