News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.


To Purchase # 2190S

2190S 10 mg $89.00.0


Find answers on our FAQs page.


PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Western blot analysis of extracts from HeLa cells untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +) or λ phosphatase (+), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower).

Learn more about how we get our images

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Nocodazole (0.1 μg/ml, 18 hr; +), using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 (upper) or NPM Antibody #3542 (lower).

Learn more about how we get our images

Chemical structure of nocodazole.

Learn more about how we get our images

Product Usage Information

Nocodazole is supplied as a lyophilized powder. For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µg/ml for 12-48 hr. Soluble in DMSO.

Storage: Store lyophilized at room temperature or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

301.3 g/mol



Molecular Formula:


Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET (5).

1.  De Brabander, M.J. et al. (1976) Cancer Res 36, 905-16.

2.  Vasquez, R.J. et al. (1997) Mol Biol Cell 8, 973-85.

3.  Jordan, M.A. et al. (1992) J Cell Sci 102 ( Pt 3), 401-16.

4.  Blajeski, A.L. et al. (2002) J Clin Invest 110, 91-9.

5.  Park, H. et al. (2012) ChemMedChem 7, 53-6.

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.