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XML generation date: 2024-10-25 17:56:09.947
Product last modified at: 2024-12-09T14:00:09.761Z
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PDP - Template Name: Chemical Modulators
PDP - Template ID: *******c501c72

Rapamycin #9904

    Product Information

    Product Usage Information

    Rapamycin is supplied as a lyophilized powder. For a 100 µM stock, resuspend 10 nmol (9.1 µg) in 100 µl ethanol or DMSO. For experiments with cultured cells, CST recommends pretreating with 10 nM of this inhibitor for one hour prior to stimulation.

    Storage

    Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 914.17 g/mol
    Purity >99%
    Molecular Formula C51H79NO13
    CAS 53123-88-9
    Solubility Soluble in DMSO at 200mg/ml and EtOH at 50mg/ml.

    Background

    Rapamycin is a bacterial macrolide with antifungal and immunosuppressant activities (1). Rapamycin forms a complex with the immunophilin FKBP12 which then inhibits the activity of FRAP/ mTOR (TOR in yeast) (2,3). Rapamycin treatment of cells leads to the dephosphorylation and inactivation of p70 S6 kinase. Rapamycin also leads to the dephosphorylation of 4E-BP1/PHAS1, thereby promoting its binding to and inactivation of eIF4E (4,5). This activity has been shown to be the basis for Rapamycin's ability to block protein synthesis and to arrest cell cycle progression in the G1-phase (6,7). However, it has been suggested that Rapamycin's inhibition of the G1/S transition may be the consequence of its effect on cyclin D1 mRNA and protein stability (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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