Chemical structure of Staurosporine.
Western blot analysis of extracts from HeLa cells, untreated or Staurosporine-treated (3 hours), showing PARP cleavage as evidence of induction of apoptosis, using Cleaved PARP Antibody #9541 (upper) or PARP Antibody #9542 (lower).
Dissolve the lyophilized alkaloid in DMSO or Methanol. For a 1 mM solution, dissolve in 0.536 mL.
Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
|Molecular Weight||466.5 g/mol|
|Solubility||Soluble in DMSO at 50mg/ml and EtOH at 2mg/ml.|
Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 μM, staurosporine induces cell apoptosis (2,3).