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9950
Trichostatin A (TSA)
Activators & Inhibitors
Chemical Modulators

Trichostatin A (TSA) #9950

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Trichostatin A (TSA): Image 1

Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2A (Lys5) Antibody.

Product Usage Information

Trichostatin A is supplied as a lyophilized powder. For a 4mM stock (10.000X), reconstitute in 826 µL DMSO. Treat cells with 400 nM trichostatin A for 12-18 hours.

Precautions: This compound is only sold for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

MW (kDa) 302.4 g/mol
Purity

>99%

Molecular Formula

C17H22N2O3

CAS 58880-19-6
Solubility Soluble in DMSO at 15mg/ml and EtOH at 3mg/ml.

Background

Trichostatin A is an antifungal antibiotic derived from Streptomyces that inhibits mammalian histone deacetylases (1,2). When used at nanomolar concentrations, trichostatin A causes the accumulation of acetylated histones in a quite specific and reversible manner (2,3). Trichostatin A has also been shown to inhibit both G1- and G2-phases of the mammalian cell cycle and has been tested for use as a potential anticancer agent (2-5).

  1. Tsuji, N. et al. (1976) J. Antibiot. 29, 1-6.
  2. Yoshida, M. et al. (1990) J. Biol. Chem. 265, 17174-17179.
  3. Kijima, M. et al. (1993) J. Biol. Chem. 268, 22429-22435.
  4. Ailenberg, M. and Silverman, M. (2002) Biochem. Biophys. Res. Commun. 298, 110-115.
  5. Maecker, H. et al. (2002) Cancer Cell 2, 139-148.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 9950S
Product # Size Price
9950S
1 mg $ 121