Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb (PE Conjugate) #29710
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb (PE Conjugate) detects endogenous levels of total Blimp-1/PRDI-BF1 protein.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1 isoform 2.
Background
Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T cells (4,5), suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13).
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- Keller, A.D. and Maniatis, T. (1991) Genes Dev 5, 868-79.
- Chang, D.H. et al. (2000) Nat Immunol 1, 169-76.
- Kallies, A. et al. (2006) Nat Immunol 7, 466-74.
- Martins, G.A. et al. (2006) Nat Immunol 7, 457-65.
- Kallies, A. and Nutt, S.L. (2007) Curr Opin Immunol 19, 156-62.
- Yu, J. et al. (2000) Mol Cell Biol 20, 2592-603.
- Gyory, I. et al. (2004) Nat Immunol 5, 299-308.
- Ancelin, K. et al. (2006) Nat Cell Biol 8, 623-30.
- Lin, Y. et al. (1997) Science 276, 596-9.
- Chen, H. et al. (2007) Mol Immunol 44, 1461-70.
- Lin, K.I. et al. (2002) Mol Cell Biol 22, 4771-80.
- Shaffer, A.L. et al. (2006) Immunol Rev 210, 67-85.
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