Render Target: STATIC
Render Timestamp: 2024-10-11T09:40:25.923Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-05-10 06:28:41.492
Product last modified at: 2024-05-30T07:05:24.014Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BST2 (E4N3W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #36756

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated BST2 (E4N3W) XP® Rabbit mAb #95940.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    BST2 (E4N3W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total BST2 protein. This clone shows a preference for the BST2 dimer by western blot.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human BST2 recombinant protein.

    Background

    BST2 (CD317, Tetherin, HM1.24) is a type II transmembrane glycoprotein functioning as a major mediator of the innate immune defense against the dissemination of enveloped viruses by tethering virion on the cell surface (1). BST2 has an N-terminal cytoplasmic tail for endocytosis and cytoskeletal signaling, a transmembrane domain, an extracellular domain containing putative disulfide bonds and coiled-coil region for homodimer formation, and a C-terminal GPI domain for membrane anchoring (2,3). Both the transmembrane domain and the GPI domain can insert either into the cell membrane or the viral envelope membrane and hold them together to prevent viral release. Some viruses encode proteins, such as HIV-1 and Vpu, respectively, to act as antagonists to counteract BST2 (2,3). BST2 is overexpressed in gastrointestinal cancers, breast cancer, lung cancer, and multiple myeloma (4-7). BST2 monoclonal antibody targeting myeloma or lung cancer cells induces cellular cytotoxicity and cell death (ADCC, antibody-dependent cell-mediated cytotoxicity). Thus, BST2 serves as a potential target for tumor immunotherapy.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.