BST2 (E4N3W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #36756
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
BST2 (E4N3W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total BST2 protein. This clone shows a preference for the BST2 dimer by western blot.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human BST2 recombinant protein.
Background
BST2 (CD317, Tetherin, HM1.24) is a type II transmembrane glycoprotein functioning as a major mediator of the innate immune defense against the dissemination of enveloped viruses by tethering virion on the cell surface (1). BST2 has an N-terminal cytoplasmic tail for endocytosis and cytoskeletal signaling, a transmembrane domain, an extracellular domain containing putative disulfide bonds and coiled-coil region for homodimer formation, and a C-terminal GPI domain for membrane anchoring (2,3). Both the transmembrane domain and the GPI domain can insert either into the cell membrane or the viral envelope membrane and hold them together to prevent viral release. Some viruses encode proteins, such as HIV-1 and Vpu, respectively, to act as antagonists to counteract BST2 (2,3). BST2 is overexpressed in gastrointestinal cancers, breast cancer, lung cancer, and multiple myeloma (4-7). BST2 monoclonal antibody targeting myeloma or lung cancer cells induces cellular cytotoxicity and cell death (ADCC, antibody-dependent cell-mediated cytotoxicity). Thus, BST2 serves as a potential target for tumor immunotherapy.
- Le Tortorec, A. et al. (2011) Viruses 3, 520-40.
- Douglas, J.L. et al. (2010) PLoS Pathog 6, e1000913.
- Kuhl, B.D. et al. (2011) J Neuroimmune Pharmacol 6, 188-201.
- Mukai, S. et al. (2016) Ann Surg Oncol, 594-602.
- Sayeed, A. et al. (2013) PLoS One 8, e67191.
- Wang, W. et al. (2009) Cancer Immunol Immunother 58, 967-76.
- Harada, T. and Ozaki, S. (2014) Biomed Res Int 2014, 965384.
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