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Render Timestamp: 2024-10-11T09:50:47.263Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-07-23 22:37:14.346
Product last modified at: 2024-07-27T08:00:10.510Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

c-Maf (E2Q5D) Rabbit mAb (Alexa Fluor® 647 Conjugate) #97118

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated c-Maf (E2Q5D) Rabbit mAb #26745.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    c-Maf (E2Q5D) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total c-Maf protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human c-Maf protein.

    Background

    Transcription factor c-Maf, also known as MAF and V-maf musculoaponeurotic fibrosarcoma oncogene homolog, is a basic leucine zipper transcription factor (bZIP) in the large Maf protein subclass and belongs to the AP-1 superfamily (1). In humans, there are three c-Maf isoforms and in mice, only two isoforms have been characterized (2). Maf proteins contain a highly conserved ancillary DNA binding domain, N-terminal to the basic domain, and bind to relatively long DNA sequences termed Maf-recognition elements (MAREs) (3). c-Maf can form homodimers and heterodimers with other bZIP proteins like Jun and Fos, as well as non-bZIP proteins (4,5). c-Maf plays an important role in lens fiber cell development, bone development, apoptosis, and the immune system (6,7). In T cells, c-Maf expression is induced by TCR stimulation, and sustained by co-stimulatory signals, and/or cytokines like IL-4, IL-6, TGF-β, and IL-27 (8-12). In macrophages, c-Maf is required for self-renewal and promotes IL-10 production while inhibiting IL-12 (13,14). c-Maf plays a critical role in intestinal immune homeostasis, regulating ILC3s, Th17 cells, γδ T cells, and regulatory T (Treg) cells (7). c-Maf is an oncogene that contributes to the progression of some hematological malignancies and is expressed in other cancers but is not always correlated with poor prognosis (15,16). 
    1. Nishizawa, M. et al. (1989) Proc Natl Acad Sci USA 86, 7711-5.
    2. Imbratta, C. et al. (2020) Front Immunol 11, 206.
    3. Kataoka, K. et al. (1994) Mol Cell Biol 14, 700-12.
    4. Kerppola, T.K. and Curran, T. (1994) Oncogene 9, 675-84.
    5. Huang, W. et al. (2002) J Biol Chem 277, 50668-75.
    6. Kawauchi, S. et al. (1999) J Biol Chem 274, 19254-60.
    7. Cosovanu, C. et al. (2022) J Exp Med 219, e20220233. doi: 10.1084/jem.20220233.
    8. Bauquet, A.T. et al. (2009) Nat Immunol 10, 167-75.
    9. Kurata, H. et al. (1999) Immunity 11, 677-88.
    10. Yang, Y. et al. (2005) J Immunol 174, 2720-9.
    11. Xu, J. et al. (2009) J Immunol 182, 6226-36.
    12. Apetoh, L. et al. (2010) Nat Immunol 11, 854-61.
    13. Soucie, E.L. et al. (2016) Science 351, aad5510.
    14. Cao, S. et al. (2002) J Immunol 169, 5715-25.
    15. Morito, N. et al. (2006) Cancer Res 66, 812-9.
    16. Uhlen, M. et al. (2017) Science 357, eaan2507. doi: 10.1126/science.aan2507.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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