Render Target: STATIC
Render Timestamp: 2024-12-02T11:54:21.364Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-20 06:17:35.768
Product last modified at: 2024-09-20T07:04:23.611Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD11c (3.9) Mouse mAb (PE Conjugate) #56025

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometric analysis in human cells.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:20
    Flow Cytometry (Live) 1:20

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD11c (3.9) Mouse mAb (PE Conjugate) recognizes endogenous levels of total CD11c protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Human

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).
    The 3.9 antibody is widely used as a marker for CD11c expression on the cell types mentioned above.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.