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42847
CD11c (3.9) Mouse mAb (redFluor™ 710 Conjugate)

CD11c (3.9) Mouse mAb (redFluor™ 710 Conjugate) #42847

APPLICATIONS

REACTIVITY SENSITIVITY MW (kDa) Isotype
H Endogenous Mouse IgG1
Flow Cytometry

Flow cytometric analysis of live human peripheral blood mononuclear cells using CD11c (3.9) Mouse mAb (redFluor™ 710 Conjugate) (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (redFluor™ 710 Conjugate) #35935 (dashed line). Analysis was performed on cells in the monocyte gate.

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Flow Cytometry

A. Solutions and Reagents

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
  2. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

B. Immunostaining

  1. Count cells using a hemacytometer or alternative method.
  2. Aliquot 0.5-1x106 cells into each assay tube (by volume).
  3. Add 2-3 ml Incubation Buffer to each tube and rinse by centrifugation. Repeat.
  4. Resuspend cells in 100 µl of diluted primary antibody (prepared in incubation buffer at the recommended dilution).
  5. Incubate for 1 hr at room temperature.
  6. Wash by centrifugation in 2-3 ml incubation buffer.
  7. Resuspend cells in the appropriate volume of PBS and analyze on flow cytometer.

posted June 2017

Protocol Id: 1504

Application Dilutions
Flow Cytometry 1:20
Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

CD11c (3.9) Mouse mAb (redFluor™ 710 Conjugate) recognizes endogenous levels of total CD11c protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Human

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

This Cell Signaling Technology antibody is conjugated to redFluor™ 710 and tested in-house for direct flow cytometry analysis in human cells.

CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).

The 3.9 antibody is widely used as a marker for CD11c expression on the above mentioned cell types.

  1. Uotila, L.M. et al. (2013) J Biol Chem 288, 33494-9.
  2. Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
  3. Siegal, F.P. et al. (1999) Science 284, 1835-7.
  4. Racine, R. et al. (2008) J Immunol 181, 1375-85.
  5. Werfel, T. et al. (1991) J Immunol 147, 2423-7.
  6. Cabañas, C. et al. (1988) Hybridoma 7, 167-76.
  7. Kristensen, J.S. et al. (1987) Blood 70, 1063-8.
Entrez-Gene Id
3687
Swiss-Prot Acc.
P20702
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
redFluor is a registered trademark of Tonbo Biosciences.

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To Purchase # 42847S

Product Number Size Price
42847S 500 µl (100 tests) $349.00.0
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