20% off purchase of 3 or more products* | Learn More >>
73359
CD11c (N418) Hamster mAb (PE Conjugate)
Antibody Conjugates

CD11c (N418) Hamster mAb (PE Conjugate) #73359

Reviews ()
Citations (0)
Filter:
  1. F

Flow cytometric analysis of live mouse bone marrow derived dendritic cells using CD11c (N418) Hamster mAb (PE Conjugate) and co-stained with MHC Class II (I-A/I-E) (M5/114.15.2) Rat mAb (APC Conjugate) #64776 (right), compared to concentration-matched Armenian Hamster Isotype Control (PE Conjugate) (left).

To Purchase # 73359S
Product # Size Price
73359S
100 µg $ 129

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Isotype Hamster (Armenian) IgG

Product Description

This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.

Application Dilutions
Flow Cytometry 1:80

Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

PRINT

View >Collapse >

Flow Cytometry

A. Solutions and Reagents

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
  2. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

B. Immunostaining

  1. Count cells using a hemacytometer or alternative method.
  2. Aliquot 0.5-1x106 cells into each assay tube (by volume).
  3. Add 2-3 ml Incubation Buffer to each tube and rinse by centrifugation. Repeat.
  4. Resuspend cells in 100 µl of diluted primary antibody (prepared in incubation buffer at the recommended dilution).
  5. Incubate for 1 hr at room temperature.
  6. Wash by centrifugation in 2-3 ml incubation buffer.
  7. Resuspend cells in the appropriate volume of PBS and analyze on flow cytometer.

posted June 2017

Protocol Id: 1504

Specificity / Sensitivity

CD11c (N418) Hamster mAb (PE Conjugate) recognizes endogenous levels of total CD11c protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).

The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9).

  1. Uotila, L.M. et al. (2013) J Biol Chem 288, 33494-9.
  2. Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
  3. Siegal, F.P. et al. (1999) Science 284, 1835-7.
  4. Racine, R. et al. (2008) J Immunol 181, 1375-85.
  5. Werfel, T. et al. (1991) J Immunol 147, 2423-7.
  6. Cabañas, C. et al. (1988) Hybridoma 7, 167-76.
  7. Kristensen, J.S. et al. (1987) Blood 70, 1063-8.
  8. Finkelman, F.D. et al. (1996) J Immunol 157, 1406-14.
  9. Sadhu, C. et al. (2007) J Leukoc Biol 81, 1395-403.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.