Render Target: STATIC
Render Timestamp: 2024-10-11T10:30:25.439Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:19:45.163
Product last modified at: 2024-09-20T07:05:33.184Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD14 (61D3) Mouse mAb (PerCP-Cy5.5® Conjugate) #99811

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometry analysis in human cells.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:20
    Flow Cytometry (Live) 1:20

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4oC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD14 (61D3) Mouse mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total CD14 protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Human

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5).
    The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Cy and CyDye are registered trademarks of GE Healthcare.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.