CD16 (3G8) Mouse mAb (PE Conjugate) #82004
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:20 |
Storage
Protocol
Specificity / Sensitivity
CD16 (3G8) Mouse mAb (PE Conjugate) recognizes endogenous levels of total CD16 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).
CD16A is expressed by NK cells, macrophages, and a subset of monocytes, while CD16B is expressed by neutrophils (4). CD16 is commonly used in combination with CD56 to characterize NK cells, with CD16 identifying NK cells capable of cytotoxicity (5).The 3G8 antibody is widely used as a marker of CD16 expression on the cell types mentioned above (6).
- Maenaka, K. et al. (2001) J. Biol. Chem. 276, 44898-44904.
- Fridman, W. H. et al. (1992) Immunol. Rev. 125, 49-76.
- Pignata, C. et al. (1993) J. Immunol. 151, 6794-6800.
- Pincetic, A. et al. (2014) Nat Immunol 15, 707-16.
- Nagler, A. et al. (1989) J Immunol 143, 3183-91.
- Fleit, H.B. et al. (1982) Proc Natl Acad Sci U S A 79, 3275-9.
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