CD3 (17A2) Rat mAb (PE Conjugate) #28306
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:40 |
| Flow Cytometry (Live) | 1:40 |
Storage
Protocol
Specificity / Sensitivity
CD3 (17A2) Rat mAb (PE Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. This antibody detects epitopes within the extracellular domains.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).
Limited Uses
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