Render Target: STATIC
Render Timestamp: 2024-12-12T10:54:14.365Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:18:32.136
Product last modified at: 2024-09-25T16:00:08.800Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD4 (RM4-5) Rat mAb (redFluor 710 Conjugate) #75508

Filter:
  • IF
  • F

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rat IgG2a kappa
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to redFluor™ 710 and tested in-house for direct flow cytometric analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.

    Application Dilution
    Immunofluorescence (Frozen) 1:200 - 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:160
    Flow Cytometry (Live) 1:160

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD4 (RM4-5) Rat mAb (redFluor™ 710 Conjugate) recognizes endogenous levels of total CD4 protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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