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Render Timestamp: 2024-11-11T09:47:40.836Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:19:28.144
Product last modified at: 2024-09-20T07:01:44.411Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD45R/B220 (RA3-6B2) Rat mAb (PE Conjugate) #93992

Filter:
  • F

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rat IgG2a kappa
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometry analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:40
    Flow Cytometry (Live) 1:40

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD45R/B220 (RA3-6B2) Rat mAb (PE Conjugate) recognizes endogenous levels of total CD45R/B220 protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).
    The RA3-6B2 clone is raised against an isoform of CD45 known as CD45R/B220. CD45R/B220 is widely used as a marker for B cells, T cell subsets, and NK cell subsets in both human and mouse (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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