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Render Timestamp: 2025-03-21T10:29:30.854Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:15:59.826
Product last modified at: 2025-03-03T21:15:14.960Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b

CD57 (HNK-1) Mouse mAb (Alexa Fluor® 647 Conjugate) #40584

Filter:
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgM
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody CD57 (HNK-1) Mouse mAb #72031.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200
    Flow Cytometry (Fixed/Permeabilized) 1:50
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. This product is stable for 12 months when stored at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD57 (HNK-1) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes the CD57 carbohydrate modification produced by the B3GAT1 protein enzyme.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells.

    Background

    CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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