|M||Endogenous||Rat IgG2a, kappa|
Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (PerCP-Cy5.5® Conjugate) and co-stained with CD44 (IM7) Rat mAb (violetFluor™ 450 Conjugate) #53289 (right), compared to concentration-matched Rat Isotype Control (PerCP-Cy5.5® Conjugate) (left).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
posted June 2017
Protocol Id: 1504
For optimal flow cytometry results, we recommend 0.25μg of antibody per test.
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
CD62L/L-Selectin (MEL-14) Rat mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total mouse CD62L protein.
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometric analysis in mouse cells.
L-selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule, responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Cy and CyDye are registered trademarks of GE Healthcare. violetFluor is a registered trademark of Tonbo Biosciences.
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|90088S||100 µg||$ 320|