Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with Cycloheximide #2112 (10 μg/ml, overnight) and mTNF-α #5178 (20 ng/ml, 4 hr; green), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (PE Conjugate).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
posted July 2009
revised June 2017
Protocol Id: 407
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (PE Conjugate) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, the unconjugated antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43) by western blot analysis.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) #8592.
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc.
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|14071S||100 µl (50 tests)||$ 356.0|