Digoxigenin (D8Q9J) Rabbit mAb (HRP Conjugate) #49620

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Digoxigenin (D8Q9J) Rabbit mAb #14682.

Product Usage Information

Application	Dilution
Western Blotting	1:20000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Protocol

Specificity / Sensitivity

Digoxigenin (D8Q9J) Rabbit mAb (HRP Conjugate) recognizes digoxigenin conjugated molecules.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated digoxigenin.

Background

Digoxigenin (DIG) is a highly immunogenic steroid derived from “foxglove” plants of the genus Digitalis (1). This small hapten molecule can be covalently linked to proteins, nucleic acids, compounds, and glycans. Antibodies targeted against the DIG hapten display high binding affinity to DIG-conjugated molecules and can be used in molecular biology assays, including in situ hybridization, western blot, ELISA, and TUNEL assays (2-4).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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