|H M R||Endogenous||Rabbit IgG|
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 (200 nM, 2 hours) and Ionomycin, Calcium Salt #9995 (3 µM, 2 hours) (green), using EGR1 (44D5) Rabbit mAb (PE Conjugate).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
posted July 2009
revised June 2017
Protocol Id: 407
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
EGR1 (44D5) Rabbit mAb (PE Conjugate) detects endogenous levels of total EGR1 protein.
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGR1 (44D5) Rabbit mAb #4154.
EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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|25357S||100 µl (50 tests)||$ 299.0|