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Folate Receptor Alpha/FOLR1 (E8U2F) Mouse mAb (Alexa Fluor® 488 Conjugate) #53873

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  • IHC

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Folate Receptor Alpha/FOLR1 (E8U2F) Mouse mAb #34265.

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:50 - 1:200

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    Folate Receptor Alpha/FOLR1 (E8U2F) Mouse mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Folate Receptor Alpha/FOLR1 protein. Non-specific staining was observed in normal human brain by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp77 of human Folate Receptor Alpha/FOLR1 protein.

    Background

    Folate Receptor Alpha (FOLR1) is a cysteine-rich cell-surface receptor that plays a critical role in the maintenance and transport of folate (vitamin B9) and reduced folic acid derivatives into cells and is essential for various cellular processes (1,2). FOLR1 binds to folate and facilitates its internalization into cells through receptor-mediated endocytosis or potocytosis and is important in cellular processes, including DNA synthesis and repair (1-3). Intracellular folate levels are critical to maintaining and supporting folate functions in its multiple coenzyme forms (4). It is primarily expressed in tissues requiring high folate levels, such as the placenta, kidneys, and certain cancer cells (5). FOLR1 has been found to be overexpressed in several types of cancer, including ovarian, lung, and breast cancer (6-8).
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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