GFP (5G4) Mouse mAb (Magnetic Bead Conjugate) #67090
- IP
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2b |
Application Key:
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:20 |
Storage
Protocol
Specificity / Sensitivity
GFP (5G4) Mouse mAb (Magnetic Bead Conjugate) detects native GFP and YFP-tagged proteins exogenously expressed in cells, but does not detect denatured GFP. This antibody does not detect RFP-tagged proteins. GFP and YFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with native GFP.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
Limited Uses
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